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anti plcb1  (Proteintech)


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    Proteintech anti plcb1
    Anti Plcb1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+plcb1/pm38778543-39-16-18?v=Proteintech
    Average 93 stars, based on 9 article reviews
    anti plcb1 - by Bioz Stars, 2026-08
    93/100 stars

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    Image Search Results


    PLCB1; a target of miR-485-3p. ( A ) Predicted binding sites between miR-485-3p and PLCB1. ( B ) Binding and mutation sites of PLCB1 3′UTR with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant PLCB1 3′UTR vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D – F ) Inhibition of PLCB1 expression by miR-485-3p at mRNA and protein levels in adipocytes. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

    doi: 10.3390/ijms25094588

    Figure Lengend Snippet: PLCB1; a target of miR-485-3p. ( A ) Predicted binding sites between miR-485-3p and PLCB1. ( B ) Binding and mutation sites of PLCB1 3′UTR with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant PLCB1 3′UTR vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D – F ) Inhibition of PLCB1 expression by miR-485-3p at mRNA and protein levels in adipocytes. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

    Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Inhibition, Expressing

    circTIAM1 as a ceRNA for miR-485-3p. ( A ) Predicted binding sites between circTIAM1 and miR-485-3p. ( B ) Binding and mutation sites of circTIAM1 with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant circTIAM1 vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D ) circTIAM1, miR-485-3p, and PLCB1 expression patterns during adipocyte differentiation. ( E ) qRTPCR analysis of mRNA of cyclin D3, CDK4, cyclin B, and PCNA expression levels. ( F ) qRTPCR analysis of mRNA of Adiponectin, C/EBPα, FABP4, and PPARγ expression levels. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

    doi: 10.3390/ijms25094588

    Figure Lengend Snippet: circTIAM1 as a ceRNA for miR-485-3p. ( A ) Predicted binding sites between circTIAM1 and miR-485-3p. ( B ) Binding and mutation sites of circTIAM1 with miR-485-3p. ( C ) Luciferase activity of wildtype and mutant circTIAM1 vectors cotransfected with miR-485-3p mimic or miR-485-3p NC into 293 T cells after 48 h. ( D ) circTIAM1, miR-485-3p, and PLCB1 expression patterns during adipocyte differentiation. ( E ) qRTPCR analysis of mRNA of cyclin D3, CDK4, cyclin B, and PCNA expression levels. ( F ) qRTPCR analysis of mRNA of Adiponectin, C/EBPα, FABP4, and PPARγ expression levels. * p < 0.05, ** p < 0.01.

    Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

    Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Expressing

    Mechanism of circTIAM1 regulation in Guangling Large-Tailed sheep adipocyte proliferation and differentiation through the miR-485-3p/PLCB1 Pathway.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

    doi: 10.3390/ijms25094588

    Figure Lengend Snippet: Mechanism of circTIAM1 regulation in Guangling Large-Tailed sheep adipocyte proliferation and differentiation through the miR-485-3p/PLCB1 Pathway.

    Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

    Techniques:

    shRNA sequences targeting PLCB1 † .

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

    doi: 10.3390/ijms25094588

    Figure Lengend Snippet: shRNA sequences targeting PLCB1 † .

    Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

    Techniques: shRNA

    qPCR primers for mRNA application.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidating the Role of circTIAM1 in Guangling Large-Tailed Sheep Adipocyte Proliferation and Differentiation via the miR-485-3p/PLCB1 Pathway

    doi: 10.3390/ijms25094588

    Figure Lengend Snippet: qPCR primers for mRNA application.

    Article Snippet: Primary antibodies used for Western blotting were cyclin D3 (1:2000, 26755-1-AP, proteintech, Wuhan, China), CDK4 (1:2000, bs-0633R, Bioss, Beijing, China), PPARG (1:2000, 16643-1-AP, proteintech, Wuhan, China), C/EBPα (1:2000, 18311-1-AP, proteintech, Wuhan, China), FABP4 (1:1000, 12802-1-AP, proteintech, Wuhan, China), Adiponectin (1:1000, bs-0471R, Bioss, Beijing, China), PLCB1 (1:1000, 26551-1-AP, proteintech, Wuhan, China), and β-actin (1:1000, bs-0061R, Bioss, Beijing, China).

    Techniques: